WebNov 12, 2024 · Apply Cytek Assay Settings for acquisition configuration and run SpectroFlo ® QC Beads as recommended by the manufacturer (see Note 9). 2. Single and unstained controls are required for spectral unmixing (see Note 10). 3. Analyze data using conventional manual gating or preferably by dimensional reduction and clustering … WebCytek® Aurora Quick Reference Guide This quick reference guide provides the basic instructions on startup, shutdown, daily QC, experiment setup, and unmixing using …
Preparing a Spectroflo Experiment - urmc.rochester.edu
Web• Prepare SpectroFlo QC beads (in the fridge in the hallway): Check if diluted bead tube from previous run is available, otherwise prepare fresh beads. Fresh beads: Shake … Webavailable throughout the SpectroFlo modules: QC & Setup • System Startup and QC: Learn how to start up the instrument and complete daily QC. • Re-Using Reference Controls: … moda phip
Cell Press: STAR Protocols
WebFor instrument support within the US, call 1-877-92-CYTEK. Visit our website, www.cytekbio.com, for up-to-date contact information. Observations Observation Possible Causes Recommended Solutions Daily QC does not complete Wrong QC bead sample Ensure you are running SpectroFlo QC beads. Bead sample not properly mixed Mix the … WebThe system also includes SpectroFlo QC beads. The cytometer is an air-cooled, compact benchtop instrument. It is equipped with three lasers and up to 48 detection channels for fluorescence, and up to two detection channels for scatter (FSC and violet laser SSC). WebJCBC Cytek Aurora . This machine is a 64 fluorescent channel Spectral Analyser. Due to this complexity, we use Spectral Unmixing instead of Compensation ... SpectroFlo QC bead lot: Evaluates machine alignment for the day, lot number must be correct: Fluorescent tags: 64 channel signatures of each dye that can be run on the machine: inmarsat telex service code ‘31’ can be used: