Human hepatocytes invitrogen
Web1 mrt. 2024 · Human intestinal epithelial cell line-6 (HIEC-6) cells and primary human hepatocytes ... and 1% penicillin/streptomycin (all purchased from Invitrogen, Thermo … Web6 mrt. 2014 · Human fetal fibroblasts (HFFs) and human adult fibroblasts (HAFs) were cultured in human fibroblast medium (HFM). HFF1 and HFF2 were derived from limbs of human fetuses. HAFs were derived from human skin biopsy. Primary human hepatocytes were purchased from Invitrogen GIBCO or Celsis In Vitro Technologies.
Human hepatocytes invitrogen
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Web6 apr. 2024 · The regenerated hepatocytes cannot be maintained and exhibit ... (1:1000, A21206, Invitrogen), donkey anti-rabbit IgG Alexa ... R. et al. Human RAD50 deficiency in a Nijmegen breakage ... Web7 apr. 2016 · To assess the features of the HLCs, primary human hepatocytes (PHs) excised from live donor liver transplantation were set as positive control. The total of 591 genes in HLCs with the fold change (FC) ≥5 (compared with HF-iPS) were grouped according to their biological process ( a ).
Web13 apr. 2024 · Functional human hepatocytes as surrogates for PHH have been generated in vitro using stem cell-based techniques, including the differentiation of human pluripotent stem cells, 12 dedifferentiation of PHH,13141516 and transdifferentiation of human fibroblasts.1718 We have successfully transdifferentiated human fibroblasts into human … Web9 uur geleden · To further investigate such a benefit and its underlying mechanisms in humans, we designed an ex vivo clinical approach to collect the circulating bioactives resulting from TOTUM-070 ingestion and to determine their …
Web8 sep. 2009 · Human hepatocytes were used here as a positive control. To detect albumin secretion, we collected culture medium supernatant samples and measured albumin … Web19 dec. 2013 · Cell lines: Immortalized Human Hepatocytes (IHH), HepG2, Hep3B (hepatoma cell lines) and HeLa-S3 (immortal cell line derived from cervical cancer cells) were cultured in DMEM (Dulbecco modified Eagle) medium glucose containing 4.5 g/L of glucose and supplemented with 10% fetal calf serum (Invitrogen, Saint Aubin, France), …
Web29 jun. 2024 · Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 ... (Invitrogen) according to the manufacturer’s instructions, using 1 µg of RNA in a 20 µL final volume and duplicates for all samples. Quantitative real-time PCRs (qPCRs) ...
WebThese '5-Donor' Human Plateable Hepatocytes are a pooled population of primary hepatocytes produced by combining cells from 5 individual donors in a single vial. … glass outdoor cylindrical light fixturesWeb12 mrt. 2024 · We purified human hepatocytes by selection with cytocidal puromycin and cultured them for more than 60 population doublings over a ... Liver tissue was shredded and incubated overnight at 37 °C in DMEM (10829–018, Invitrogen; Thermo Fisher Scientific, Inc., MA, USA) containing 0.25% Collagenase Type I (17018029, Gibco ... glass outdoor dining table setsWeb11 sep. 2024 · Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) hold great promise in toxicological applications as well as in regenerative … glass outdoor light fixturesWebPrimary human hepatocytes are widely used for basic research, pharmaceutical testing, and therapeutic concepts in regenerative medicine. Human hepatocytes can be isolated … glass outdoor heater in italyWeb1 dag geleden · Wang et al. developed a clinical-grade bioartificial liver device with cryopreserved GMP-compliant human-induced hepatocytes (hiHep-BAL). hiHep-BAL treatment improved functions and regeneration in pigs with post-hepatectomy liver failure and increased their survival. Moreover, a clinical study demonstrated the feasibility and … glass outerwrapglass outdoor railingsWeb9 mrt. 2024 · Additionally, using CellNet, we analyzed reprogrammed human induced hepatocytes (hiHeps) 12 and public RNA-seq data of conventional iPSC-derived … glass outdoor fish tank