Witryna1 sty 2006 · Locked nucleic acids (LNA) show remarkable affinity and specificity against native DNA targets. Effects of LNA modifications on mismatch discrimination were studied as a function of sequence context and identity of the mismatch using ultraviolet (UV) melting experiments. A triplet of LNA residues centered on the … WitrynaThe invention claimed is: 1. A method for detecting a target nucleic acid in a sample, said method comprising contacting said sample with: a first oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that …
Locked nucleic acid: modality, diversity, and drug discovery
Witryna4 mar 2015 · Novel attributes of Locked Nucleic Acid (LNA) makes it preferable over most of the other classes of modified nucleic acid analogues and therefore, it has been extensively explored in different synthetic oligonucleotide based therapeutics. In addition to five oligonucleotides of this class undergoing clinical trials, a healthy pipeline in pre … Witryna1 lip 2003 · Recently, we have described a new microarray platform based on the use of locked nucleic acid (LNA) oligonucleotides, a novel class of bicyclic RNA analogs having an exceptionally high affinity toward their complementary DNA and RNA target molecules (16– 18). Spotting of LNA-substituted oligonucleotides onto expression … pantone001c
Exosome-mediated delivery of functionally active miRNA-142-3p …
WitrynaOver the last decade, single stranded oligonucleotides (ON) have gained increased attention as a new drug modality. Because the assessment of genotoxicity risk during … WitrynaLocked Nucleic Acid is a novel type of nucleic acid analog that contains a 2'-O, 4'-C methylene bridge ( Figure 1 ). This bridge–locked in the 3'-endo … WitrynamM ethylenediaminetetraacetic acid (EDTA). All three ONs were mixed in similar amounts and further diluted in deionized water prior to use. Figure 2. Schematic of phosphorothioated locked nucleic acid. Phophorotioate: a non-bridging oxygen is replaced by a sulfur (red). Locked nucleic acid: the 2’ oxygen is linked to the 4’ carbon. エンバーゴ