WebThese results support the concept that PTEN loss tends to occur as a subclonal event within a given established prostatic carcinoma clone after ERG gene fusion. The combination of ERG and PTEN IHC staining can be used as a simple test to ascertain PTEN and ERG gene rearrangement status within a give … WebApr 12, 2024 · IHC staining for Ki-67, androgen receptor (AR), vimentin, PTEN, and programmed death-ligand 1 (PD-L1) were performed on unstained 4-µm thick tissue sections that had been prepared from a representative core biopsy paraffin block of the original untreated tumor, as previously described .

Immunohistochemistry and Next Generation Sequencing are

WebJan 1, 2016 · In the present study, PTEN IHC staining, with a validated protocol, of a mixed-tumor TMA with 10 tumor types and a prostate-tumor TMA with 82 cases illustrates that it is difficult to make definite pathological interpretations of PTEN expression either for reasons of biological complexity or because of preanalytical variables such as length of fixation, … WebPTEN IHC staining. PTEN IHC staining was carried out using a rabbit monoclonal antibody against PTEN (1:50 dilution, Y184; Epitomics, Burlingame, CA, USA). PTEN immunoreactivity was assessed based on cytoplasmic staining by using a semiquantitative scoring method that divided the samples into four categories as follows: ... gdgw maintenance

PTEN (138G6) Rabbit mAb Cell Signaling Technology

WebEndometrial carcinomas show frequent PTEN-PI3K pathway abnormalities, and there are currently multiple trials focused on PI3K pathway inhibitors in endometrial carcinoma … WebJul 26, 2024 · IHC-based assessment of PTEN status has been shown to correlate tightly with genomic alterations of the PTEN locus and captures not only loss of the gene, but also mutation and epigenetic changes that lead to PTEN functional inactivation [4, 9, 10] and the potential clinical utility of PTEN IHC as a valuable prognostic marker has been … WebOct 14, 2011 · PTEN is a tumor suppressor commonly inactivated in prostate cancer. Genomic deletions of PTEN are associated with poor prognosis, but are interrogated in … gdgws sohna fee payment